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High-Performance Green Extraction of Natural Products

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Nguyễn Gia Hào

Academic year: 2023

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Use of deep eutectic solvents to prepare mixed extracts of three long-lived trees with maximized skin-related bioactivities. Finally, in their informative review, Detsi and Skarpalezos [6] tried to summarize the use of deep eutectic solvents for the extraction of flavonoids, one of the most important classes of plant secondary metabolites. Effect of deep eutectic solvents on the extraction of polyphenols from grape seeds and skins.Appl.

Use of deep eutectic solvents to prepare mixed extracts of three long-lived trees with maximized skin-related bioactivities.Appl.

Green Extracts from Coffee Pulp and Their

Application in the Development of Innovative Brews

  • Introduction
  • Materials and Methods 1. Chemicals and Reagents
  • Results and Discussion 1. Extraction Yield
  • Conclusions

The yield in total polyphenols (YTP) was expressed as mg gallic acid equivalents (GAE) per g of coffee pulp weight. Beers prepared from coffee grounds (AQC and AQC/CD) were compared with beers obtained from coffee pulp (AQCW and AQCW/CD). There is currently very little published research on the development of functional beverages from coffee grounds.

However, the effect of using coffee by-products on the sensory profile of the functional drinks was not investigated.

Table 1. Experimental values and coded levels of the independent variables used for the central composite design
Table 1. Experimental values and coded levels of the independent variables used for the central composite design

Impact of Deep Eutectic Solvents on Extraction of Polyphenols from Grape Seeds and Skin

Materials and Methods 1. Standards and Reagents

After one hour of incubation at room temperature in the dark, the absorbance of blue staining was measured at 630 nm against a blank sample on an MTP reader (BIOTEK, USA, ELx800 Absorbance Microplate Reader). Diluted samples and Trolox solutions (20μL) were added together with FRAP working solution (280μL) in 96-well microplate in triplicate. Diluted samples and standard solutions (7μL) were allowed to react with 193μL of the DPPH radical solution (1.86×10−4mol L−1DPPH in ethanol, ex tempore prepared) in 96-well microplate in triplicate.

The method was performed as follows: diluted samples and Trolox solutions (20 μL) were added to 280 μL of ABTS radical solution in MTP wells in triplicate.

Table 2. Parameters of linear regression, LOD and LOQ for phenolic compounds by HPLC analysis.
Table 2. Parameters of linear regression, LOD and LOQ for phenolic compounds by HPLC analysis.

Results and Discussion 1. Total Polyphenol Content (TPC)

Despite the similar polarity and acidity of the extraction solvents, differences were observed between acidified aqueous ethanol and ChCit selectivity. Different lowercase letters (a-f) indicate significant differences between solvents and varieties (p≤0.05) based on Tukey's post hoc test. Different lowercase letters (a-e) indicate significant differences between solvents and varieties (p≤0.05) based on Tukey's post hoc test.

Flavan-3-ol major composition and antioxidant activity of seeds from different grape cultivars grown in Turkey. Eur.

Figure 1. Total polyphenol content of seed extracts obtained from ten grape varieties
Figure 1. Total polyphenol content of seed extracts obtained from ten grape varieties

Development of a Low-Temperature and

High-Performance Green Extraction Process for the Recovery of Polyphenolic Phytochemicals from Waste

Results and Discussion

The assessment of the fitted model and the suitability of the response surface was accomplished by performing ANOVA and a lack of fit test (Figure 2), based on the closeness of measured and predicted values ​​(Table 2). The 3D diagrams representing the model (Figure 3) visualize the effect of the process variables on the response (YTP). The optimum pH 5 determined by the model was the upper limit of the experimental design, suggesting that extraction was not favored in a more acidic environment.

However, the carboxyl group of the quinine moiety on CGA, which is the main polyphenol in PP [5], has a pKaof 3.33 and at pH 5 this group would be deprotonated [23]. Also, careful regulation of SS results in effective dispersion of the solute diffusing from the interior of the solid particles into the bulk of the liquid. In support of this finding were the results of another study on the interactions of stilbene (E)-picethanol with HP-β-CD, where a decrease in the complexation constant was observed in response to an increase in T[40].

In order to better demonstrate the effect of HP-β-CD on polyphenol extraction from PP, extracts obtained under optimized conditions at 30 °C were tested for antioxidant activity. Four phytochemicals, namely neochlorogenic acid, chlorogenic acid, caffeic acid and ferulic acid, could be tentatively identified among the traces recorded at 325 nm (Figure 5). Under the optimized conditions, the total polyphenol yield and antioxidant properties of the produced extracts were comparable to those obtained with aqueous methanol and ethanol.

Some of the main polyphenols identified in the extracts were neochlorogenic acid, chlorogenic acid, caffeic acid and ferulic acid. This article is an open access article distributed under the terms of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Figure 2. Desirability function (A), actual-by-predicted plot (B) and statistical information (lack-of-fit and parameter estimates inset tables) generated by performing response surface methodology, concerning the effect of process variables on the response
Figure 2. Desirability function (A), actual-by-predicted plot (B) and statistical information (lack-of-fit and parameter estimates inset tables) generated by performing response surface methodology, concerning the effect of process variables on the response

The Effect of Ultrasonication Pretreatment on the Production of Polyphenol-Enriched Extracts from

Materials and Methods 1. Chemicals

Evaluation of the fitted models and response surface suitability was based on the ANOVA test and lack of fit test. R2 provides an indication of the amount of total variability around the mean that is explained by the regression model. The impact of the ultrasonication pretreatment can be more characteristically depicted by the changes found in the optimization settings of all three variables.

Optimization of the extraction process revealed significant changes in extraction variables as a consequence of pretreatment with ultrasound, but to portray the effect of ultrasound on the process in an integrated framework, information related to extraction kinetics and the effect of temperature was indispensable. In all cases examined, fitting the kinetic model to the experimental data gave R2>. The effect exerted by ultrasound pretreatment was shown to be temperature dependent, as extraction acceleration (k increase) was seen only at 70 and 80 ◦C.

This assumption could justify the fact that at temperatures of 40–60◦C, the stirred tank extraction of the ultrasound-pretreated samples proceeded at a slower rate. On the other hand, the non-pretreated samples had a higher content of polyphenols on the outside of the solid particles and apparently showed increased kandh. The Ea values ​​for the extractions without and with ultrasonication pretreatment were 11.24 and 34.02 kJ mol−1, respectively (Table 6), clearly demonstrating that the extraction of the ultrasonication-pretreated samples required almost 4 times more energy.

However, such an observation could be quite misleading and the increase in YTP(s) of the ultrasonicated samples should not be overlooked. Kinetics showed that a higher extraction rate, and thus a shorter extraction time, of the ultrasound-pretreated samples could only be achieved when extraction was performed in a stirred tank at T≥70◦C.

Table 1. Process (independent) variables used for the experimental design and their codified levels.
Table 1. Process (independent) variables used for the experimental design and their codified levels.

Application of Deep Eutectic Solvents to Prepare Mixture Extracts of Three Long-Lived Trees with

Results and Discussion 1. Preparation of DESs

Because CC had the highest ISO level among the three trees, it was therefore used as a model for comparing the extraction efficiency of the prepared DESs. DES 10, with yields similar to those of DES 8 and 9, was excluded from further consideration due to its low purity of lactic acid (∼90%). Next, the effect of the water content of DES 1 was evaluated by measuring the ISO yields with DES 1 at different water contents (10–50% w/w).

However, it is noteworthy that the extraction efficiency of the selected solvent was achieved without any heat treatment, and that the individual DES components can act as functional ingredients in cosmetics [20]. These factors may provide merit for the use of the DES solution in this study. The fit of the linear, quadratic and special cubic models for the three responses was analyzed (Table S3).

For the anti-elastase activity, only a special cubic model was significant, of which the linear terms, the quadratic term X2X3 and the cubic term were significant (Table S4). The skin-beneficial bioactivities of the mixtures of extracts from three kinds of long-lived trees were investigated to identify potential new cosmetic product materials. Different mixtures of the three leaf extracts were prepared and systematically analyzed according to the simplex-centroid mixture design, which provided information on the effects of the individual leaves on the three different bioactivities, as well as the optimal compositions of the three leaves for maximized antioxidant, anti-tyrosinase and anti-elastase activities.

Chromatographic profiles of three extracts of (a) Ginkgo biloba, (b) Cinnamomum camphora and (c) Cryptomeria japonicaleave prepared using 70% w/w DES 1. Multi-response optimization of the extraction solvent system for phenolics and antioxidant activities by a simple design flemoroidy.F.

Table 2. Simplex-centroid mixture design and the resulting responses.
Table 2. Simplex-centroid mixture design and the resulting responses.

Deep Eutectic Solvents as Extraction Media for Valuable Flavonoids from Natural Sources

Factors Affecting the Extraction of Flavonoids Using DES Separation Techniques

In extractions, it is reported that the polarity of DES is a very important factor affecting solubility. When screening multiple DES against many samples and target compounds, the conclusion implies that the polarity of the DES used must be close to the polarity of the target substance. Any competitive interaction between the ingredients of the DES and another presence in the system can interfere with the extraction efficiency to a great extent, potentially leading to a redesign of the extraction [23,24].

Reducing their presence in the DES reduced the yield simply because of the decrease in interaction between the less chloride anions and the target compound [20]. However, in the in vivo tests, the NaDES showed higher toxicity than the DES, a result attributed to the higher viscosity of the NaDES. The usually high viscosity of DES or NaDES is the major drawback that can limit their use as extraction solvents, as it hinders penetration of the solvent into the extraction matrix.

The concentration of water in the DES-water or NaDES-water mixture affects the efficiency of the extraction, as has been shown by Bi et al. 40] showed that the viscosity of DES is affected by the water content and that if more than 50% water is present, the hydrogen bonding structure of the DES components is destroyed. Higher water content tends to break the hydrogen bonding structure of DES, reducing its effectiveness.

The pH level of the system can in some cases dictate the shape of the target compound, which eventually affects its solubility in DES. Whenever deemed necessary, often due to the extraction methodology, some researchers chose to perform filtration or centrifugation prior to conventional HPLC analysis to confirm the extraction of the target flavonoids [53].

Conclusions

Green and Efficient Extraction of Four Bioactive Flavonoids from Pollen Typhae by Ultrasound-Assisted Deep Eutectic Solvent Extraction.J. Development of hydrophobic deep eutectic solvents for pesticide extraction from aqueous environments.Fluid Phase Equilib. Optimized Extraction of Bioactives from Herba Artemisiae Scopariae with Ionic Liquids and Deep Eutectic Solvents.J.

Evaluation of new natural deep eutectic solvents for the extraction of isoflavones from soy products. Talent. Simultaneous extraction of flavonoids from Chamaecyparis obtusaUsing deep eutectic solvents as solvent additives of conventional extractions.J. Evaluation of deep eutectic ternary solvents as new tools for the extraction of flavonoids from Ginkgo biloba. September.

Deep eutectic solvents as a green medium for the extraction of flavonoid glycosides and aglycones from Platycladi Cacumen.J. Efficient extraction of major catechins in Camellia sinensis leaves using deep eutectic solvents based on green choline chloride. RSC Adv. Extraction of phenolic compounds from virgin olive oil with deep eutectic solvents (DES).Food Chem.

Improved extraction of phenolic compounds using choline chloride based deep eutectic solvents from Juglans regiaL.Ind. Polyol-based deep eutectic solvents for extraction of natural polyphenolic antioxidants from Chlorella vulgaris.ACS Sustain.

Hình ảnh

Table 4. Developed attributes and references standards in the sensory evaluation.
Table 5. Mean intensity scores of aroma and taste attributes for the different brews.
Figure 2. Spider graph for the sensory attributes of the different brews. (a) AQC: aqueous extract from coffee seeds, (b) AQC/CD: β-cyclodextrin extract from coffee seeds, (c) AQCW: aqueous extract from coffee pulp, (d) AQCW/CD: β-cyclodextrin extract from coff
Table 1. Characteristics of grape varieties.
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